U2OS cell line stably expressing Adenosine A2B Receptor and cAMP Biosensor

Description U2OS cell line stably expressing Adenosine A2B Receptor and cAMP Biosensor cAMP Gentaur Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Gentaur Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP Gentaur Adenosine A2B Receptor Cell Line contains U2OS cells stably expressing the following constructs: Human Adenosine A2B receptor (ADORA2B) with no tag cAMP Gentaur Biosensor (Red Fluorescence) cAMP Gentaur ADORA2B Receptor Cell Line allows for the assay of compounds that modulate the G-protein signaling pathway via Gs protein activation. When an agonist binds to ADORA2B, the Gs protein is activated, which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase in the red fluorescence intensity of the cAMP biosensor. Thus, the activity can be easily quantified in living cells in a dose-response manner by fluorescence emission in a microplate reader.